Testing for Biocompatibility

A bound antimicrobial must be biocompatible with the tissue it is intended to contact.  To prove this, Orthobond applies multiple biocompatibility assays for each surface it is screening.  Some of these assays are shown below.  In addition to these assays, Orthobond has successfully taken several candidate technologies through various ISO 10993-1 tests (data on file at Orthobond).

cell staining

Cell Staining assay. Rhodamine phalloidin with DAPI counterstain is used to label cells which are seeded onto biomaterial surfaces. In the images above, human osteoblasts are seen one day after being seeded onto stainless steel Caption: (316L) surfaces. It is apparent that the treatment shown above has no harmful effect on attachment or spreading. At Orthobond, cell staining studies are often performed at 1, 4 and 8 days. Scale bar is 100 microns.



safety MTS assay.  Mitochondrial activity is used to determine relative cell viability over multiple time points.  In the data shown to the right, human osteoblasts grown on control and treated titanium surfaces were assayed by MTS at 1, 4 and 8 days.  The treated surface showed no interference in cell attachment or proliferation. Error bars are standard deviation, n=4 for all time points.