Screening for Efficacy

Many antimicrobials will lose their antibacterial activity when bound to a surface. Orthobond performs unique and rigorous testing to find and validate suitable candidates. Novel candidate surfaces must demonstrate antimicrobial efficacy in multiple surface-based assays. A few examples of the testing used in our screening process are shown below.

 

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Spray Method. A chromatography sprayer is used to deposit bacteria onto treated surfaces. A film of agar is then cast over the surface and is assessed for bacterial colony growth. Actual experimental examples of control untreated (top) and treated (bottom) surfaces are shown above to the right.

 

 

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ASTM 2149. Treated surfaces are placed into flasks containing bacteria and incubated with vigorous shaking. As bacteria contact the surface they are exposed to bound antimicrobials. After a specified contact time the bacteria are plated on petri dishes. In the results shown to the right, a treated surface (red bars) was challenged for 48 hours including re-exposure to fresh bacteria after 24 hours. The % remaining is shown (error bars are standard deviation, n=3 for all time points).